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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

Copper accumulates in the senile plaque where it is tightly bound to Abeta ( In vitro , the Abeta-Cu 2+ complex, in the presence of a reducing agent, reduces oxygen to produce H 2 O 2 , and this involves recycling copper from Cu 2+ to Cu 1+ and back to Cu 2+ along with the oxidation of the reducing agent ( 2+